215 Spatial transcriptomics on human epidermal equivalents

Single-cell RNA seq analyses on human epidermis have given new insights into cell fates of the tissue by characterizing precise transcriptomic profile at single-cell scale, but information spatial localizations transcripts in each single cells is lacking. 3D are therefore great importance to characterize with resolution. To study epidermal biology and diseases, organotypic models can be grown using keratinocytes, which reproduce architecture epidermis. These equivalent (3D HEE) allow modelling related diseases caused genetic defects as well environmental signals insults. However, they need further validated singe level demonstrate that recapitulate vivo biology. Here we applied a novel technology HEEs. This based synthetic aperture optics cyclical single-molecule fluorescence in-situ hybridization, enabling up 30 genes per experiment. Using panel characterized marker for different layers, detected expression these expected showing designed probe set able We also included known involved cycle phases, aiming test this detecting rare Notably, use MKI67, markers proliferating cells, clear few cycling stratum basale. thus show models. powerful technique used dissect HEEs space affected or cues.